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恒远文献:人肿瘤坏死因子α(TNF-α)ELISA试剂盒引用文献

发布时间:2020/8/14浏览:973次

【文献标题】 The distinct role of CD73 in the progression of pancreatic cancer

【作者】Liangjing Zhou1,Shengnan Jia1,Yan Chen,et.al

【作者单位】浙江大学(Zhejiang University)

【文献中引用产品】

人肿瘤坏死因子α(TNF-α)ELISA试剂盒

【关键词】Pancreatic cancer,Progression,CD73,TNFR2,miR-30a-5p

【DOI】doi.org/10.1007/s00109-018-01742-0

【影响因子(IF)】4.14

【出版期刊】 《Journal of Molecular Medicine》

【产品原文引用】 

Detection of TNF-α levels in supernatant via ELISA

Supernatant was investigated for levels of TNF-α protein using commercial TNF-α ELISAs (Hengyuan Biotechnology Co., Ltd., Shanghai, China) according to the manufacturer’s instructions.

Western blot

Cells were harvested, and total protein lysates were resolved using 10% sodium dodecyl sulfate–polyacrylamide gels,electroblotted onto a polyvinylidene fluoride membrane,blocked by 5% skim milk, and probed with primary antibodies listed in Table S1. The membrane was then incubated with the secondary antibody and visualized by enhanced chemiluminescence using Kodak X-Omat LS film (Eastman Kodak,Rochester, USA).

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